Cell proliferation in the ectoderm of the Xenopus embryo: development of substratum requirements for cytokinesis |
| |
| Authors: | R Winklbauer |
| |
| Institution: | 1. College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China;2. Zhejiang Fisheries Technical Extension Center, Hangzhou, Zhejiang, China;1. Department of Pediatric Surgery, Saitama Medical University, Saitama, Japan;2. Department of Pediatric Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;3. Division of Tissue engineering, The University of Tokyo Hospital, Tokyo, Japan;4. Biotube Co., Ltd, Osaka, Japan;1. College of Medicine, University of Arkansas for Medical Sciences, 4301W. Markham Street, Slot # 720, Little Rock, AR 72205, USA;2. Professor, Chief of Plastic Surgery Division, Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, AR, USA |
| |
| Abstract: | The requirements for cell division in ectodermal blastomeres of the early Xenopus embryo were studied. Isolated blastomeres divide autonomously on nonadhesive agar in a simple salt solution up to the midblastula stage. After the midblastula transition, cell-cell contact is required for blastomere division. In isolated blastomeres of that stage, cytokinesis fails, but nuclear division continues normally for some time. Cell-cell contact as a prerequisite for blastomere division can be replaced by culturing blastomeres on an appropriate substratum. Clonal growth of isolated blastomeres is supported by a variety of protein substrata, indicating rather unspecific substratum requirements. Different substrata which do not support blastomere division can affect different steps in cytokinesis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
