The effect of seed treatment with benzimidazole-based fungicides on infection of the foliage of overwintered salad onions by Botrytis cinerea

Seed treatments of carbendazim (Bavistin 50% W.p.) and thiophanate-methyl (Mildothane 50% W.p.) applied to overwintered salad onions at 250 g a.i./kg seed protected the foliage of plants from infection by Botrytis cinerea during the seasons 1973–1976. Crop establishment and yield were also improved. Seed treatment with calomel was not effective. Chemical analysis of treated non-viable seeds, retrieved from the soil, indicated that 73% carbendazim and 46% thiophanate-methyl remained attached to the seeds after 9 months in the soil. Analyses of onion leaves revealed that each fungicide was represented by similar quantities of carbendazim, 5 μg/g fresh weight in October 1975 reducing to 1 μg/g fresh weight in May 1976. Bioassay tests showed that the fungicide was acropetally distributed and was present in all leaves early in the season (October) but was absent from some new leaves formed in the following spring. Carbendazim-insensitive isolates of B. cinerea occurred after three seasons' use of this chemical. Sensitive isolates failed to grow on agar containing 1 μg/ml benomyl but all insensitive isolates (31 total) grew normally at this concentration and some were capable of growth on agar containing 1000 μg/ml benomyl. The emergence of foliar isolates of the fungus insensitive to the benzimidazole-based compounds used in the treatment of seeds indicated that these fungicides did not provide a permanent solution to the disease problem.