Precultivation technique for studies of microorganisms exhibiting overflow metabolism |
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Authors: | Hop Thi Bich Pham Gen Larsson Sven-Olof Enfors |
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Institution: | (1) Department of Biotechnology, Royal Institute of Technology, S- 100 44 Stockholm, Sweden |
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Abstract: | A precultivation technique for microorganisms exhibiting overflow metabolism is presented. It is based on a low initial medium volume in the fermenter. The medium feed contains all the nutrients, but is diluted with respect to sugar, the concentration of which determines the biomass concentration at the end of the preculture. By this method, effects of varying activity of the inocula from shake flask cultures are minimised and the metabolic state, i.e. oxido-reductive, oxidative growth on sugar plus overflow metabolite or oxidative growth on sugar alone, can be controlled at the start of the main fermentation. |
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Keywords: | precultivation cultivation overflow metabolism Saccharomyces cerevisiae |
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