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Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling |
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| Authors: | Anna U?arowska Giuseppe Dionisio Barbara Sarholz Hans-Peter Piepho Mingliang Xu Christina R?nn Ingvardsen Gerhard Wenzel Thomas Lübberstedt |
| Affiliation: | (1) Department of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350 Freising, Germany;(2) Faculty of Agricultural Sciences, University of Aarhus, Department of Genetics and Biotechnology, Research Centre Flakkebjerg, Slagelse, DK-4200, Denmark;(3) General Motors Powertrain Germany GmbH, 65423 R?sselsheim, Germany;(4) Department of Bioinformatics, University of Hohenheim, Fruwirthstrasse 23, 70593 Stuttgart, Germany;(5) National Maize Improvement Center of China, China Agricultural University, 2 West Yuanmingyuan Road, Beijing, 100094, PR, China;(6) Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen,;(7) Department of Agronomy, Iowa State University, 1204 Agronomy Hall, 50011 Ames, Iowa, USA |
| Abstract: | BackgroundThe potyviruses sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are major pathogens of maize worldwide. Two loci, Scmv1 and Scmv2, have ealier been shown to confer complete resistance to SCMV. Custom-made microarrays containing previously identified SCMV resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecular mechanisms involved in maize-potyvirus interactions. |
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