| 桑木层孔菌液体培养过程中几种胞外酶活性的变化 |
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| 引用本文： | 曹春蕾,崔宝凯,秦问敏.桑木层孔菌液体培养过程中几种胞外酶活性的变化[J].菌物学报,2011,30(2):275-280. |
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| 作者姓名： | 曹春蕾 崔宝凯 秦问敏 |
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| 作者单位： | 1. 北京林业大学微生物研究所,北京,100083
2. 中国科学院沈阳应用生态研究所,沈阳,110016 |
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| 基金项目： | 中央高校基本科研业务费专项资金（No. BLYX200912）；国家自然科学基金（No. 30870013, 30910103907） |
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| 摘 要： | 研究了桑木层孔菌Phellinus mori液体培养过程中发酵液中淀粉酶、果胶酶、羧甲基纤维素酶（CMC酶）和漆酶等4种胞外酶的活性变化，同时测定了蛋白质、还原糖和pH的变化。结果表明，桑木层孔菌拥有丰富的胞外酶系，淀粉酶酶活第7天时达到最大值，果胶酶在第9天和第12天的酶活都较高，羧甲基纤维素酶第10天时酶活最高，漆酶酶活第9天时达到最大值，说明桑木层孔菌对淀粉类物质利用最早。蛋白质浓度在第8天和第11天时出现两个峰值，还原糖浓度随培养时间逐渐降低，发酵液pH值在培养初期逐渐变小，后期逐渐变大。
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| 关 键 词： | 淀粉酶 果胶酶 羧甲基纤维素酶 漆酶 酶活 |
Activity changes of several extracellular enzymes in liquid culture of Phellinus mori |
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| Authors: | CAO Chun-Lei CUI Bao-Kai and QIN Wen-Min |
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| Institution: | Institute of Microbiology, Beijing Forestry University, Beijing 100083, China;Institute of Microbiology, Beijing Forestry University, Beijing 100083, China;Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China |
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| Abstract: | Phellinus mori had rich extracellular enzymes. The amylase activity in liquid culture of the fungus had the maximum value on day 7. Pectinase showed two peaks on day 9 and 12, respectively. The maximum value of carboxymethyl cellulose enzyme (CMCase) activity appeared on day 10. The laccase reached its peak on day 9. It was indicated that Phellinus mori preferred starch to pectin, cellulose and lignin. The protein concentration had two peaks on day 8 and 11, respectively. The reducing sugar concentration decreased gradually with the incubation time, and the pH value decreased on days 2-7 but increased after day 8. |
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| Keywords: | amylase pectinase carboxymethyl cellulose enzyme laccase enzyme activity |
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