Pretreatment and saccharification of rice hulls for the production of fermentable sugars |
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Authors: | Gong-Yuan Wei Wa Gao II-Hyuck Jin Su-Young Yoo Jai-Heon Lee Chung-Han Chung Jin-Woo Lee |
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Institution: | (1) School of Life Science, Soochow University, Suzhou, 215123, China;(2) BK21 Bio-Silver Project, Dong-A University, Busan, 604-714, South Korea;(3) Department of Biotechnology, Graduate School, Dong-A University, Busan, 604-714, South Korea |
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Abstract: | Hydrolytic conditions of rice hulls by acid and alkaline treatments before enzymatic saccharification were optimized in this
study. Based on the results of single-factor experiments and an orthogonal array experiment, reaction time was found to be
the most important factor for the acidic hydrolysis of rice hulls. Maximum yield of sugars from 1 g of rice hulls by acidic
treatment under optimized conditions was 213.6 mg. The yield of lignin removal from acidic pretreated rice hulls by alkaline
treatment increased with increase in reaction temperature and time. The amount of sugars obtained from 1 g of pretreated rice
hulls by enzymatic saccharification was 307.7 mg, and the conversion rate of sugars from crude fibers in pretreated rice hulls
was about 72%. Instrumental analyses with FTIR and SEM indicated that lignin in rice hulls was partially removed by alkaline
treatment, and the structure of rice hulls became deformed and more fibers were exposed to cellulases after acidic treatment. |
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