细菌血红蛋白基因在产D-阿拉伯糖醇酵母菌中的克隆与表达

构建了含透明颤菌血红蛋白基因vgb和甲醛抗性基因SFA1的重组质粒pVgb EX2 ,转化到产D 阿拉伯糖醇的酵母Saccharomycessp.X 62中。转化子细胞中VHb的含量比对照菌细胞有显著提高 ,表明基因vgb在酵母细胞中得到表达。转化子发酵的D 阿拉伯糖醇产量与转化率均有提高。在重复实验中D 阿拉伯糖醇的产量最多提高了 2 7 3%。在实验条件下 ,似乎D 阿拉伯糖醇的产量与细胞VHb含量有相关性。

CLONING AND EXPRESSION OF VHB GENE IN D-ARABITOL PRODUCING YEAST

Recombinant plasmid pVgb\|EX2 containing Vitreoscilla hemoglobin gene vgb and formaldehyde resistant gene SFA1 was constructed and transformed into D\|arabitol producing yeast strain Saccharomyces sp.X\|62.The fact that the amount of VHb in transformant cells was considerably higher than that in control cells indicated that gene vgb was expressed in transformant cells.D\|arabitol productivity and yield of fermentation by transformants were improved.The most improvement of D\|arabitol productivity in repeat experiments reached 27.3%.It appeared that the fermentation productivity of D\|arabitol was relative to the amount of VHb in cells under experimental conditions.