Utilization of an alternative carbon source for efficient production of human alpha(1)-antitrypsin by genetically engineered rice cell culture |
| |
Authors: | Terashima M Ejiri Y Hashikawa N Yoshida H |
| |
Institution: | Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1-1, Gakuen-cho, Sakai, 599-8531, Osaka Japan. terasima@chemeng.osakafu-u.ac.jp |
| |
Abstract: | Human alpha(1)-antitrypsin was produced by genetically engineered rice cells using promoter and signal peptide of a rice alpha-amylase isozyme. Batch and continuous cultures were employed to investigate the effects of alternative carbon sources on the alpha(1)-antitrypsin production. While this expression system is inducible by sugar depletion, we have found that the productivity of alpha(1)-antitrypsin increased 2.4- to 3.4-fold, compared with the control medium without carbon source, in medium containing an alternative carbon source, such as pyruvic acid and glyoxylic acid. The accumulated alpha(1)-antitrypsin in the medium containing pyruvic acid reached 18.2-24.2 mg/g-dry cell in 50-70 h by batch culture. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|