A Peptide Motif from the Second Fibronectin Module of the Neural Cell Adhesion Molecule,NCAM, NLIKQDDGGSPIRHY,is a Binding Site for the FGF Receptor |
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Authors: | Jacob Jacobsen Vladislav Kiselyov Elisabeth Bock Vladimir Berezin |
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Institution: | (1) Protein Laboratory, Department of Neuroscience and Pharmacology, Panum Institute, University of Copenhagen, Building 24.2, Blegdamsvej 3, 2200 Copenhagen N, Denmark;(2) Present address: Hagedorn Research Institute, Niels Steensens Vej 6, 2820 Gentofte, Denmark |
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Abstract: | The mechanism of fibroblast growth factor receptor (FGFR) activation by the neural cell adhesion molecule (NCAM) is not well
understood. A motif in the second NCAM fibronectin type III (FN3) module, termed FGL, has by means of nuclear magnetic resonance
(NMR) and surface plasmon resonance (SPR) analyses been demonstrated to be involved in NCAM–FGFR interactions. An FGFR activation
motif (FRM) in the first NCAM FN3 module also has been suggested to take part in NCAM interactions with FGFR. Here, we show
for the first time that a peptide motif in the second NCAM FN3 module, different from the previously described FGL motif (NLIKQDDGGSPIRHY;
termed BCL) binds and activates FGFR and induces FGFR-dependent neurite outgrowth in cultures of cerebellar granule neurons.
Our results provide evidence that the BCL motif is one of the multiple FGFR binding sites in NCAM.
Special issue article in honor of Dr. Anna Maria Giuffrida-Stella. |
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Keywords: | NCAM FGF receptor Peptide motif Neurite outgrowth Surface plasmon resonance |
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