Optimum culture conditions for the production of benzonitrilase by Rhodococcus rhodochrous J1 |
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Authors: | Toru Nagasawa Michihiko Kobayashi Hideaki Yamada |
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Institution: | (1) Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, 606 Kyoto, Japan |
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Abstract: | We sought the optimum conditions for the production of benzonitrilase by Rhodococcus rhodochrous J1. The use of isovaleronitrile or isobutyronitrile as an inducer greatly enhanced benzonitrilase formation. When Rhodococcus rhodochrous J1 was cultivated at 28°C for 96 h in a medium consisting of 0.1 ml of isovaleronitrile, 0.5 g of polypeptone, 0.3 g of malt extract, 0.3 g of yeast extract and 1 g of glycerol per 100 ml of tap water (pH 7.2), and isovaleronitrile was fed twice at the concentrations of 0.1% (v/v) and 0.2% (v/v) at 55 h and 77 h, respectively, during the course of cultivation, the enzyme activity in the culture broth reached approximately 3,100-times higher than the initially obtained level. |
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Keywords: | Benzonitrilase Isovaleronitrile Rhodococcus rhodochrous J1 Induction |
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