Increasing l‐isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two‐component export system BrnFE |
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| Authors: | L Yin F Shi X Hu C Chen X Wang |
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| Institution: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, , Wuxi, China;2. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, , Wuxi, China |
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| Abstract: | Aims To increase the l ‐isoleucine production in Corynebacterium glutamicum by overexpressing the global regulator Lrp and the two‐component export system BrnFE. Methods and Results The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW‐8 individually or in combination. The constructed plasmids were transformed into an l ‐isoleucine‐producing strain C. glutamicum JHI3‐156, and the l ‐isoleucine production in these different strains was analysed and compared. More l ‐isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in l ‐isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, l ‐isoleucine production in JHI3‐156/pDXW‐8‐lrp‐brnFE increased 63% in flask cultivation, and the specific yield of l ‐isoleucine increased 72% in fed‐batch fermentation. Conclusions Both Lrp and BrnFE are important to enhance the l ‐isoleucine production in C. glutamicum. Significance and Impact of the Study The results provide useful information to enhance l ‐isoleucine or other branched‐chain amino acid production in C. glutamicum. |
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| Keywords: | BrnFE
Corynebacterium glutamicum
l‐isoleucine production Lrp pDXW‐8 |
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