Genome-wide identification and resistance expression analysis of the <Emphasis Type="Italic">NBS</Emphasis> gene family in <Emphasis Type="Italic">Triticum urartu</Emphasis> |
| |
Authors: | Jing Liu Linyi Qiao Xiaojun Zhang Xin Li Haixian Zhan Huijuan Guo Jun Zheng Zhijian Chang |
| |
Institution: | 1.College of Graduate,Shanxi University,Taiyuan,China;2.Shanxi Key Laboratory of Crop Genetics and Molecular Improvement/Institute of Crop Science,Shanxi Academy of Agricultural Sciences,Taiyuan,China |
| |
Abstract: | As the largest class of resistant genes, the nucleotide binding site (NBS) has been studied extensively at a genome-wide level in rice, sorghum, maize, barley and hexaploid wheat. However, no such comprehensive analysis has been conducted of the NBS gene family in Triticum urartu, the donor of the A genome to the common wheat. Using a bioinformatics method, 463 NBS genes were isolated from the whole genome of T. urartu, of which 461 had location information. The expansion pattern and evolution of the 461 NBS candidate proteins were analyzed, and 118 of them were duplicated. By calculating the lengths of the copies, it was inferred that the NBS resistance gene family of T. urartu has experienced at least two duplication events. Expression analysis based on RNA-seq data found that 6 genes were differentially expressed among Tu38, Tu138 and Tu158 in response to Blumeria graminis f.sp.tritici (Bgt). Following Bgt infection, the expression levels of these genes were up-regulated. These results provide critical references for further identification and analysis of NBS family genes with important functions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|