X-ray-induced mutation to 6-thioguanine resistance in cultured human diploid fibroblasts |
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Authors: | Roger Cox WK Masson |
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Institution: | M.R.C. Radiobiology Unit, Harwell, Didcot, Oxon. OX11 ORD England |
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Abstract: | X-ray induced mutation to 6-thioguanine (6TG)-resistance was studied in early passage cultures of human diploid fibroblasts.The appearance of phenotypic induced mutants in irradiated cell populations was linearly related to the number of post-irradiation cell doublings and to the duration of the growth period prior to mutant selection; the maximum yield of X-ray induced mutants was observed when cells surviving radiation had completed 3–4 doublings (6–7 days growth_in non-selective medium.The maximum induced mutation frequency was linearly related to X-ray dose and the mutation rate was estimated to be 3.1 · 10?7 mutations per viable cell per rad.The data obtained for X-ray induced mutations in cultured human diploid fibroblasts were compared with (a) similar experimental data obtained with established cell cultures and (b) theoretical predictions of X-ray mutation rates in human germ cells. |
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