The causal deletions in the second exon of <Emphasis Type="Italic">An-3</Emphasis> closely associated with awn development and rice yield |
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Authors: | Lilong Liu Yunzhen Li Minyi Wei Zishuai Wu Fang Liu Yongfu Qiu Jijing Luo Rongbai Li Baoxiang Qin |
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Institution: | 1.Agricultural College,Guangxi University,Nanning,People’s Republic of China;2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Nanning,People’s Republic of China;3.Guangxi Colleges and Universities Key Laboratory of Plant Genetics and Breeding,Nanning,People’s Republic of China;4.College of Life Science,Guangxi University,Nanning,People’s Republic of China;5.Rice Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,People’s Republic of China |
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Abstract: | Awn is one of important traits during rice domestication. To understand the development of rice awn and the roles it played in rice domestication, we preliminary mapped a major QTL An-3 for awn development using chromosome segment substitution line CSSL138 developed by introgressed genomic fragments of long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) into genetic background of short-awned indica cultivar 93–11. An-3 was then fine mapped to a 7-kb region of chromosome 8. An epidermal patterning factor-like protein gene was identified as the single candidate gene corresponding to this QTL. An-3 was showed to be an allele of RAE2 and GAD1, and negatively regulated 1000-grains weight, grain length, and length–width ratio. Comparing with the coding sequences of An-3 from CSSL138, a 2- and 4-bp frame-shift deletions in the second exon were identified in 93–11 and Nipponbare, respectively. Taken together, our results provide valuable natural variation in the alleles of An-3 between common wild rice and cultivated rice, which will be helpful in clarifying the mechanism of awn development and promoting the application of an-3 in genetic improvement of rice yield traits. |
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