Ribohomopolymer formation as a source of error in the radioactive precursor incorporation studies of nuclear DNA-dependent RNA synthesis |
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Authors: | Fu-Li Yu |
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Affiliation: | Department of Biochemistry, Jefferson Medical College Thomas Jefferson University Philadelphia, Pennsylvania 19107, U.S.A. |
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Abstract: | Isolated rat liver nuclei contain ribohomopolymer polymerases with relative activities in the following order: Poly (A) (100%) > Poly (C) (62%) > Poly (U) (34%) > Poly (G) (13%). Because these enzymes share the same substrates with the nuclear DNA-dependent RNA polymerases in nuclei, labelled precursor is therefore concurrently incorporated into both RNA and ribohomopolymer. Thus, experiments designed to study DNA-dependent RNA synthesis are subjected to error. It is estimated when [14C]ATP is used as the labelled precursor, the error is as high as 35%; [14C]CTP, 20%; [14C]UTP or [14C]GTP, 10%. |
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