Modulatory effect of fenugreek saponins on the activities of intestinal and hepatic disaccharidase and glycogen and liver function of diabetic rats |
| |
Authors: | Khaled Hamden Bassem Jaouadi Tahia Salami Serge Carreau Samir Bejar Abdelfattah Elfeki |
| |
Affiliation: | (1) Biotechnology High School of Sfax (ISBS), Soukra Km 4.5, P.O. Box 261, Sfax, 3052, Tunisia;(2) Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, P.O. Box 95, Sfax, 3052, Tunisia;(3) Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax, 3018, Tunisia;(4) Laboratory of Histology–Embryology, Faculty of Medicine of Sfax, Sfax, 3029, Tunisia;(5) Department of Biochemistry, University of Caen-Basse Normandie, USC INRA, EA2608 CHU-Caen, France |
| |
Abstract: | Diabetes mellitus is a serious health concern throughout the world and is often associated with a variety of bodily disorders such as liver toxicity and dysfunction. This study elucidates the effect of fenugreek saponin administration on disaccharidase and glycogen activities in the intestine and liver of surviving diabetic rats. It also evaluates the effect of saponin feeding using a number of liver toxicity indices, namely stress oxidant, liver dysfunction markers and metabolism. Our findings indicate that the fenugreek saponin fraction significantly modulated the disaccharidase and glycogen enzyme activities in the intestine and liver of rats, increased the hepatic glycogen content, suppressed the increase of blood glucose level and improved results in the oral glucose tolerance test (OGTT). The fenugreek saponin extract also efficiently protected the hepatic function, which was evidenced by the significant increases of superoxide dismutase (SOD), catalase (CAT), gluthation peroxidase (GPX), aspartate transaminase (AST), alanine transaminase (ALT) and lactate deshydrogenase (LDH) enzyme activities. Fenugreek saponin also induced a notable delay in the absorption of LDL-cholesterol and triglycerides and a remarkable increase in levels of HDL-cholesterol. A histological analysis of the hepatic tissues further established the positive effect of fenugreek saponin. Overall, the findings of the current study indicate that fenugreek saponins exhibit attractive properties and can be considered as promising candidates for future application as therapeutic agents in biotechnological and bioprocess-based technologies, particularly those related to the development of anti-diabetic, hepatoprotective and hypolipidemic drugs. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|