New in vitro reporter gene bioassays for screening of hormonal active compounds in the environment |
| |
Authors: | Kateřina Svobodová Tomáš Cajthaml |
| |
Institution: | (1) Laboratory of Environmental Biotechnology, Institute of Microbiology of the ASCR, v.v.i., Videnska, 1083 Prague, Czech Republic |
| |
Abstract: | Identification of chemicals with endocrine-disrupting activities in the past two decades has led to the need for sensitive
assays for detection and monitoring of these activities in the environment. In vitro reporter gene assays represent a relatively
fast and easy-to-perform method for detection of compounds that are able to bind to hormonal receptors and stimulate or silence
their transactivation activity, thus interfering with the hormone signaling pathways. This paper reviews upgrades on reporter
gene assays performed during the last decade. The utilization of new reporter genes (luciferase and green fluorescent protein
coding genes) significantly improved the sensitivity of the tests and made them faster. Reporter gene assays now represent
a high-throughput system for screening chemicals for hormonal activity. Finally, modification of test set-ups for testing
anti-hormonal activities also enabled measurements of endocrine-disrupting activities in complex environmental samples such
as sediments and wastewater treatment plant effluents. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|