High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system |
| |
| Authors: | Teresa Corral Lorena S Ver Geneviève Mottet Olga Cano Blanca García-Barreno Lesley J Calder John J Skehel Laurent Roux José A Melero |
| |
| Institution: | 1.Centro Nacional de Microbiología,Instituto de Salud Carlos III,Majadahonda,Spain;2.Department of Microbiology and Molecular Medicine,University of Geneva Medical School, CMU,Geneva,Switzerland;3.National Institute for Medical Research, The Ridgeway,London,UK |
| |
| Abstract: | Background Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers.
However, eggs have found so far only limited use in the production of recombinant proteins. We now describe a system, based
on a Sendai virus minigenome, to produce large amounts of heterologous viral glycoproteins in the allantoic cavity of embryonated
eggs. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
