Activation and inhibition of spinach ribulose 1,5-bisphosphate carboxylase by 2-phosphoglycolate |
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Authors: | A.S. Bhagwat |
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Affiliation: | Biology and Agriculture Division, Bhabha Atomic Research Centre, Bombay 400 085, India |
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Abstract: | Ribulose 1,5-bisphosphate carboxylase when activated by preincubation with 1 mM bicarbonate and 10 mM magnesium chloride can be further activated ca 20–500% by incubating with 2.5 mM phosphoglycolate depending upon the pH of the preincubation medium. The activation effects were seen only under specific preincubation conditions. The activation by phosphoglycolate was a slow reaction requiring ca 15 min for maximal effect. Even though magnesium was essential for phosphoglycolate activation, concentrations higher than 15 mM progressively inhibited the activation of the enzyme by phosphoglycolate. When added directly to the reaction mixture, phosphoglycolate was a potent inhibitor of the carboxylase activity. Even under preincubating conditions, phosphoglycolate showed slight inhibitory effect at 0.1 mM and activation was observed at concentrations higher than 0.5 mM. The KA value for phosphoglycolate was 2.8 mM. |
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Keywords: | Chenopodiaceae spinach ribulose 1,5-bisphosphate carboxylase phosphoglycolate inhibition product activation. |
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