Factors affecting the extraction and the relative proportions of multiple forms of plant α-galactosidases |
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Authors: | PM Dey MJ Hustler JB Pridham N Sumar |
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Institution: | Department of Biochemistry, Royal Holloway College, University of London, Egham Hill, Egham, Surrey, TW20 0EX, U.K. |
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Abstract: | The extractability of α-galactosidase activity from mature Vicia faba seeds and the conversion of the low molecular weight form II to the larger oligomer I, was examined over a range of salt concentrations. Specific and total activities of the preparations were high when strong salt solutions were used. Extraction of α-galactosidase I, in comparison with II, requires solutions with a high ionic strength (e.g. 0.5 M NaCl). Interpretation of gel filtration patterns are, however, complicated by conversion of II to I which occurs under these conditions. This conversion is also enhanced by routine procedures used for enzyme purification, such as citric acid precipitation. |
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Keywords: | Leguminosae broad beans α-galactosidases multiple forms |
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