Surface trapping and AFM detection of DNA topological intermediates generated from an oxidative chemical nuclease |
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| Authors: | Mukhopadhyay R Srivatsan S G Verma S |
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| Affiliation: | Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India. rupam@iitk.ac.in |
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| Abstract: | Direct observation of DNA topological intermediates generated from a 'chemical nuclease' treatment has been made by atomic force microscopy (AFM). The intermediates were trapped at the mica-water interface and imaging was carried out in the dynamic force mode. Complete conversion from supercoiled circular state to relaxed circular/linear state has been observed over a time scale of 8 min. Implication of such studies in complementing gel electrophoresis data has been predicted. |
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| Keywords: | AFM DNA Oxidative cleavage Chemical nuclease Single molecule |
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