Salmonella enteritidis temperature-sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes |
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Authors: | Gherardi M M Gómez M I García V E Sordelli D O Cerquetti M C |
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Institution: | Departamento de Microbiología Aplicada, Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Serrano 669, 1414 Buenos Aires, Argentina. |
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Abstract: | The protection conferred by temperature-sensitive mutants of Salmonella enteritidis against different wild-type Salmonella serotypes was investigated. Oral immunization with the single temperature-sensitive mutant E/1/3 or with a temperature-sensitive thymine-requiring double mutant (E/1/3T) conferred: (i) significant protection against the homologous wild-type Salmonella strains; (ii) significant cross-protection toward high challenge doses of S. typhimurium. Significant antibody levels against homologous lipopolysaccharide and against homologous and heterologous protein antigens were detected in sera from immunized mice. Moreover, a wide range of protein antigens from different Salmonella O serotypes were recognized by sera from immunized animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized Salmonella antigens from different serotypes. Taken together, these results indicate that temperature-sensitive mutants of S. enteritidis are good candidates for the construction of live vaccines against Salmonella. |
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Keywords: | Salmonella enteritidis vaccine Temperature-sensitive mutant Cross protection Salmonella typhimurium |
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