从水稻根部悬浮培养细胞分离原生质体及植株再生

以长香稻(Oryza sativa L．)(糯稻)的幼嫩种子根为材料，在Ms和N6培养基上诱导产生结构松软而分散的愈伤组织，经过AA液体培养基振荡悬浮培养，悬浮培养细胞经酶解后得到了活性较高的原生质体，试验结果表明这是分离原生质体较理想的材料。在附加2，4-D lmg／L(以下单位同)、KT(激动素)0．2、各氨酰胺876、天门冬酰胺266的Ms琼脂糖培养基中，诱导出了大量愈伤组织，在含KT2、NAA(α-萘乙酸)0．2、zT(玉米素)0．2、cH(水解酪蛋白)1000和4％椰子汁的N6培养基中成功地诱导出了由原生质体再生的植株。当代原生质体再生植株能正常开花、结实、产生种子；染色体数均为二倍性(2n＝24)，最显著的特点是结实率低，穗粒数减步、生育期延迟。

Protoplast Isolation from Root Suspension Culture Cells and Plant Regeneration in Rice

After loose calli are produced from tender roots Oryza sativa L. on MS and N6 media, much active protoplasts are obtained by means of protoplasts isolated from callus-derived shock suspension culture in an agitated liquid AA medium. The experiment shows that this is an ideal material to isolate protoplasts . Calli from protoplasts are developed in MS agarose medium containing 2, 4-D Img/L (the following unit is the same ), KT 0. 2, NAA 0. 2, glutamine 867; asparagine 266. Plantlets are induced on N6 medium containing KT 2, NAA 0. 2, CH 1000, 4% coconut milk. The plants generated from protoplast R! generation are able to bloom, produce and seed normally; the number of chromosomes is diploidy (2n = 24). The most characteristic of all is the low rate of the seed setting, reduction in the number grains of each spike and the prolonged time of growth .