Influence of Exogenous Hormones on Somatic Embryogenesis in Hevea brasiliensis |
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Authors: | EL HADRAMI I; CARRON M P; D'AUZAC J |
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Institution: | *Laboratory of Applied Plant Physiology, University of Montpellier 2 F-34095 Montpellier, Cedex 5
In vitro Culture Laboratory, Institut de Recherches sur le Caoutchouc IRCA-CIRAD, BP 5035, F-34032 Montpellier, Cedex, France |
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Abstract: | Generating somatic embryos from the inner teguments of Heveaseeds is difficult. Like other ligneous plants, the rubber-treeis generally considered to be recalcitrant with regard to somaticembryogenesis. In this paper we show that when conventionallyused amounts of both 3, 4-dichlorophenoxyacetic acid (3, 4-D)and benzylaminopurine (BAP) are reduced by 24 times andwhen the length of exposure to these hormones is also reduced,the embryogenic capacity of calli increases without affectingcallogenesis. The germination of several embryos and plantletswas obtained. We conclude that an excess of auxin and cytokininin the medium, even of a weak auxin such as 3, 4-D, and theprolonged exposure to these hormones suppress the embryogeniccapacity of the callus. Hevea brasiliensis, rubber-tree, somatic embryogenesis, hormones |
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