SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS |
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| Authors: | Y Peng Loh Y Sarne M P Daniels H Gainer |
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| Institution: | Behavioral Biology Branch, National Institute of Child Health and Human Development, Bethesda, MD 20014, U.S.A.;Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20014, U.S.A. |
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| Abstract: | Abstract— Neurosecretory cells (bag cells and R3–14 neurons) in the abdominal ganglion of Aplysia californica were 'pulse-chased' in 3H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude 'neurosecretory granule' fraction on acid-urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post-translational processing of the neurosecretory proteins occurs intragranularly (L oh et al , 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae. |
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