高脂饮食诱导的C57BL／6J肥胖小鼠脂肪组织RIP140基因表达水平的研究

目的：探讨高脂饮食致肥胖小鼠脂肪组织RIP140mRNA表达水平的变化及其与胰岛素抵抗的关系。方法：将C57BL／6J雄性小鼠随机分为正常饮食（NFD）组、高脂饮食（HFD）纽分别喂养14周后，测量两组小鼠体重，以NFD组小鼠体重作为对照，选取HFD组中体重大于对照组小鼠平均体重20％的小鼠作为肥胖组小鼠。对照组和肥胖组小鼠取血测甘油三酯（TG）、总胆固醇（TC）、空腹血糖（FBG）、空腹胰岛素水平（FIns），计算稳态模型胰岛素抵抗指数（HOMA-IR）；采用RT—PCR技术检测两组小鼠附睾脂肪组织RIP140 mRNA的表达水平，并进行统计学分析。结果：HDF组小鼠中有12只符合标准计入肥胖组。肥胖组小鼠TG、TC、FBG、Fins（P〈0．05），HOMA-1R（P〈0．01）均明显高于对照组；肥胖组小鼠脂肪组织RIP140mRNA的表达高于对照组，差异具有统计学意义（P〈0．05）；相关分析显示小鼠脂肪组织R1P140 mRNA表达水平与TG水平呈正相关（r=0．536，P〈0．05），与胰岛素抵抗指数呈正相关（r=0．465，P〈0．05），而与TC、FBG、Fins水平相关分析无统计学意义（P〉0．05）。结论：高脂饮食诱导的肥胖小鼠脂肪组织RIP140 mRNA表达增加，并与胰岛素抵抗程度呈正相关。

Research on the Expression Level of RIP 140 Gene in Adipose Tissue of High Fat Diet-induced C57BL/6J Obesity Mice

Objective： To study the expression level of RIP140mRNA in adipose tissue of high fat diet-induced obese mice and the correlation with insulin resistance. Methods： Male C57BL/6J mice were divided into two groups： normal-fat diet group （NFD） and high-fat group （HFD）, fed respectively for 14 weeks. Mice in HFD group whose body weight were 20 % higher than average weight of NFD mice were selected as obese mice. The triglycerides （TG）, total cholesterol （TC）, fast blood glucose （FBG）, fast insulin （Fins） levels of the NFD group and obese mice were assayed and the HOMA-IR were calculated. The expression of RIP140 mRNA in epididymal adipose tissue was examined by RT-PCR. Results： A total of 12 mice in HDF group were recruited into the obesity group. The level of TG, TC, FBG, Fins （P〈0.05）,HOMA-IR （P〈0.01）in obesity group were higher than those of control group; The expression level of RIP140 mRNA in adipose increased significantly in obesity group （P〈0.05）, when compared with the controls. The correlation analysis showed that the expression of RIP140 mRNA in mice adipose were positively correlated with HOMA -IR（r=0.465, P〈0.05） and TG（r=0. 536,P〈0. 05 ）, but not with TC, FBG and Fins. Conclusions： The expression level of RIP140 mRNA in adipose tissue is increased and positively correlated with insulin resistance in mice fed with high fat diet.