The influence of Trisenox on actin organization in HL-60 cells |
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| Authors: | Magdalena Izdebska Dariusz Grzanka Lidia Gackowska Agnieszka Żuryń Alina Grzanka |
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| Affiliation: | (1) Department of Histology and Embryology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland;(2) Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland;(3) Department of Immunology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland |
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| Abstract: | The aim of this study was to show the influence of Trisenox (arsenic trioxide, ATO) on cytoplasmic and nuclear F-actin organization in HL-60 human leukemia cell line. Changes in localization were determined with the use of fluorescence microscopy and flow cytometry. Alterations, in both cytoplasmic and nuclear actin, were observed in cells exposed to ATO. F-actin network underwent accumulation and formed aggregates, that were very often placed under the cell membrane in whole cells and at the periphery of isolated nuclei. Addition of ATO also induced apoptosis and a decrease in G2 phase cells. These results suggest the influence of actin on the formation of apoptotic bodies and also participation of this protein in apoptotic alterations within nuclei, i.e. chromatin reorganization. |
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| Keywords: | F-actin HL-60 Trisenox (arsenic trioxide, ATO) Apoptosis Fluorescence Flow cytometry |
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