Prototheca paracutis sp. nov., a novel oleaginous achlorophyllous microalga isolated from a mangrove forest |
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| Institution: | 1. Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand;2. Microbe Division/Japan Collection of Microorganisms (JCM), RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan;3. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand;1. Laboratory, Biological Business Department, Ichimasa Kamaboko Co., Ltd., 77-1 Junishin, Agano-shi, Niigata, 959-1936, Japan;2. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;3. Graduate School of Sustainability Science, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;1. The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;2. Laboratory, Biological Business Department, Ichimasa Kamaboko Co., Ltd., 77-1 Junishin, Agano-shi, Niigata, 959-1936, Japan;3. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;1. Laboratorio de Patología Vegetal, INIA Treinta y Tres, Ruta 8 Km 281, CP 33000, Treinta y Tres, Uruguay;2. Center for Forest Mycology Research, Northern Research Station, U.S. Forest Service, Madison, WI, 53726-2398, USA;3. Laboratorio de Micología, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo, Uruguay;1. Programa de Pós-Graduação em Sistemática e Evolução, Universidade Federal do Rio Grande do Norte, Campus Universitário Natal, 59072-970, Brazil;2. Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte, Campus Universitário Natal, 59072-970, Brazil;3. Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo 2, Madrid, Spain |
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| Abstract: | Two strains of yeast-like achlorophyllous alga belonging to the genus Prototheca were isolated from the water and soil, respectively, from a mangrove forest in Thailand. Cultures of both strains were achlorophyllous when grown under light and dark conditions, and they reproduced by release of sporangiospores. Phylogenetic analyses, based on the D1/D2 region of the large subunit ribosomal RNA gene and the small subunit ribosomal RNA gene nucleotide sequences, revealed these two strains were closely related to Prototheca cutis. The strains grew well at 25 °C, weakly or slowly at 30 °C, but not at 35 °C and higher, and were found to be susceptible to 50 μg/disk clotrimazole, as determined by the disk diffusion test. They assimilated a limited number of carbon/nitrogen compounds; glucose, galactose, trehalose, ethanol, glycerol, propylene glycol, lactic acid, fructose and mannose as sole sources of carbon, and ammonium, lysine and cadaverine as sole sources of nitrogen. The two strains are clearly distinguished from P. cutis by the abilities to assimilate polypropylene glycol and the inability to grow at 35 °C and higher. In this study, the ninth member of the genus Prototheca, Prototheca paracutis sp. nov. (ex-type strain YMTW3-1T = JCM 32112T = TBRC8745T), is proposed. The MycoBank number is MB 821626. In addition, P. paracutis sp. nov. was observed to accumulate lipid at up to 21% of the cell dry weight, characterizing it as an oleaginous microorganism. |
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| Keywords: | Oleaginous microorganism Yeast-like microalga Physiology |
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