Characterisation of proteolytic enzymes of Eurygaster integriceps Put. (Sunn bug), a major pest of cereals |
| |
| Institution: | 1. Department of Agicultural Entomology, All-Russian Research Institute of Plant Protection (VIZR), 3, Podbelsky shosse, Pushkin, St. Petersburg 196608, Russia;2. Department of Microbiological Plant Protection, All-Russian Research Institute of Plant Protection (VIZR), 3, Podbelsky shosse, Pushkin, St. Petersburg, 196608, Russia;3. Department of Biochemistry and Molecular Biology, Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42-44, B.Morskaya Street, St. Petersburg 190000, Russia;4. Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK;1. National Museums of Scotland Collection Centre, 242 West Granton Rd, Edinburgh EH5 1JA., Scotland, UK;2. Bogor Agricultural University, Kampus IPB, Dramaga Bogor, 16680, West Java, Indonesia;1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Center for Biological Control, Florida A&M University, Tallahassee 32307, FL, United States;4. Entomology and Nematology Department, University of Florida, Gainesville 32611, FL, United States;5. Department of Entomology, China Agricultural University, Beijing 100193, China;1. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;2. Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea;3. Division of Crop Protection, National Institute of Agricultural Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea;1. Ilsong Institute of Life Science, Hallym University, Anyang 14066, Republic of Korea;2. Department of Agricultural Biology, Seoul National University, Seoul 08826, Republic of Korea;3. Department of Biomedical Gerontology, Hallym University, Chuncheon 24252, Republic of Korea;4. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;5. Laboratory of Pine Wilt Disease, Division of Forest Insect Pests & Disease, National Institute of Forest Science, Seoul, 02455, Republic of Korea;1. School of Forest Resources and Conservation, University of Florida, Gainesville, FL, USA;2. Technical Center of Zhuhai Inspection & Quarantine, Gongbei Customs District P. R. China, Zhuhai, China;3. Institute of Plant Protection, Shanghai Academy of Landscape Architecture Science and Planning, Shanghai, China;1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, PR China;2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China;3. Kaihua County Animal Husbandry and Veterinary Bureau, Quzhou 324300, PR China |
| |
| Abstract: | Eurygaster integriceps (Sunn pest or Sunn bug) is one of the most significant pests of wheat and is responsible for substantial losses in yield and quality of wheat grain in Europe and Asia. Sunn pest salivary gland-derived proteases and other hydrolases damage grain proteins and starch. Characterisation of protease activities from both Sunn pest salivary glands and Sunn pest-damaged wheat grains revealed a broad range of activities in terms of substrate specificity and diversity of isoelectric point. Neutral and alkaline proteases present in Sunn pest-damaged grains were shown to be capable of hydrolyzing gluten proteins, whilst some proteases were also shown to be active against gelatin. The neutral serine proteases present play the dominant role in degradation of gluten quality. The sensitivity of some proteases to proteinaceous and non-proteinaceous serine proteinase inhibitors was shown, including that of a recombinantly expressed protease. It was found that proteases isolated from Sunn pest salivary glands could be activated by trypsin indicating that they are present as zymogens in vivo. Analysis of individual Sunn pest-damaged grains showed great diversity in the proteases present. This work highlights the challenges of developing proteinase inhibitors to manage Sunn pest damage. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
