Freezability and Fertility Results with Uncentrifuged Stallion Semen |
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Authors: | Heiskanen M-L Hilden L Hyyppä S Kangasniemi A Pirhonen A Mäenpää P H |
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Institution: | 19.Department of Biochemistry & Biotechnology, University of Kuopio, P.O. Box 1627, SF-70211, Kuopio, Finland ;29.Equine Research Station, Agricultural Research Centre, Ypäjä, Finland ; |
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Abstract: | The first (1 to 3) sperm-rich fractions of the ejaculate were collected from 4 stallions using an open-ended vagina. The volume of the collected fractions was 12 ± 8 ml with a density of 475 ± 200 million spermatozoa/ml. Before freezing, the semen was diluted with a skim-milk based extender 1:1 to 1: 8 (volume of semen: volume of extender), depending on the initial sperm concentration to achieve a final concentration of 100 million/ml. The total number of spermatozoa in an insemination dose ranged from 0.7 to 1 billion spermatozoa. Within 12 h after ovulation, 48 mares were inseminated in 70 cycles. The total single-cycle pregnancy rate at day 21 was 24%, but varied from 10% to 33% per cycle among the stallions. |
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