Effect of pyridalyl on mortality,fecundity and physiological performance of olive fruit fly,Bactrocera oleae Rossi (Diptera: Tephritidae) |
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| Affiliation: | 1. Department of Plant Protection, University Campus2, University of Guilan, Rasht, Iran;2. Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran;3. Department of Agricultural Entomology Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.;1. Department of Zoology, Government Arts College, Melur, 625106 Madurai, Tamil Nadu, India;2. Centre for Research in Aquatic Entomology, The Madura College, Madurai 625011, Tamil Nadu, India;3. Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Rajasthan 305801, India;1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China;2. Department of International Cooperation and Exchange, Southwest University of Science and Technology, Mianyang, China;3. Department of Entomology, College of Agriculture, South China Agricultural University, Guangzhou, China;1. Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan;2. Laboratory of Creative Science for Insect Industries, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan;3. Laboratory of Sanitary Entomology, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan;4. Laboratory of DNA Replication and Recombination, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 4700 KAUST Thuwal, 23955, Jeddah, Saudi Arabia;1. Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Vietnam;2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Nghia Do, Cau Giay, Hanoi, Vietnam |
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| Abstract: | Toxicity and physiological effects of pyridalyl were studied on the adults of Bacterocera oleae via oral exposure and biochemical experiments. First, adults were fed on a protein hydrolysate diet which has been amended by five concentrations of pyridalyl. Then, another cohort of flies was fed on LC50 concentration to find potential effects on fecundity, detoxifying enzymes and intermediary metabolism. Results of bioassay showed a concentration of 0.517 μg/ml as the LC50 value with confidence limit of 0.305–0.791 μg/ml at 95%. Fecundity of the females fed on pyridalyl-treated diet showed no significant differences with controls following 7 days of exposure while a significant higher mortality was recorded in laid eggs by the treated females. Activities of esterase and glutathione S-transferase significantly increased in the treated adults compared to control when α-naphthyl acetate and CDNB (1-chloro-2,4-dinitrobenzene) were used as substrates. Activities of alanine- and aspartate aminotransferases significantly increased in the pyridalyl-treated adults compared to control while no significant difference was observed in activity of ɤ-glutamyl transferase. Although lactate dehydrogenase showed no statistically different activity but both acid- and alkaline phosphatases had statistically higher activity in the control adults compared to treatment. Finally, low density lipoprotein and total protein were the only non-enzymatic components which showed statistical difference between control and treated flies. Our results indicated that pyridalyl had toxicity on B. oleae and intervened in the physiological performance of flies. |
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