Complete mitochondrial genome of Dacus conopsoides (Insecta: Tephritidae) with tRNA gene duplication and molecular phylogeny of Dacini tribe |
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| Institution: | 1. China-ASEAN College of Marine Sciences, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor, Malaysia;2. Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;4. Faculty of Science and Mathematics, Mataram University, Mataram, Indonesia;5. Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;1. Department of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran;2. Department of Plant Protection, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran;1. Oak Ridge Institute for Science and Education, USA;2. USDA-ARS, Pacific Basin Agricultural Research Center, Hilo, HI, USA;3. Institute of Life Science, Gyeongsang National University, Jinju, Gyeongsangnam-do, South Korea;4. Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk-do, South Korea;5. USDA-ARS, National Biological Control Laboratory, Stoneville, MS, USA;1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, PR China;2. College of Plant Protection, Hunan Agricultural University, Changsha, Hunan 410128, PR China;3. Entomological Laboratory, Kyushu University, Graduate School of Bioresource and Bioenvironmental Sciences, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;4. Entomological Laboratory, Kyushu University, Faculty of Agriculture, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan |
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| Abstract: | To date there is only a single report on the complete mitochondrial genome of the Dacus fruit flies. We report here the whole mitogenome of Dacus conopsoides with first report of tRNA gene duplication in tephritid fruit flies determined using next-generation sequencing and discuss the molecular phylogeny of Dacini tribe. It had a total length of 15,852 bp, comprising 13 protein coding genes, 2 rRNA genes, 23 tRNA genes, and a non-coding region (A + T-rich control region). The 65-bp trnF gene was duplicated, and the 68-bp trnE gene was partially duplicated resulting in a 31-bp pseudogene. The cloverleaf structure for trnN, trnH, and trnF lacked the TΨC-loop, while trnS lacked the D-stem. The start codons for the protein coding genes included 6 ATG, 3 ATC, 2 ATA, and 1 each of ATT and TCG. Seven PCGs had TAA stop codon, two had TAG and four had incomplete T stop codon. Molecular phylogeny based on 15 mt-genes (13 PCGs +2 rRNA genes) and 30 taxa of Tephritidae indicated D. conopsoides forming a monophyletic sister group with D. longicornis supported by high bootstrap value. The lineage containing also the monophyletic genus Zeugodacus. The Dacini and Ceratitidini tribes of the subfamily Dacinae were monophyletic but the subfamilies Dacinae and Trypetinae were paraphyletic. A broader taxa sampling of the Tephritidae is needed to better elucidate the phylogenetics and systematics of the tribes and subfamilies of tephritid fruit flies. |
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