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Immunocytochemical Localization of Aromatase in The Ovary of Superovulated Cattle,Pigs and Sheep |
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| Authors: | Lautincik J Kolodzieyski L Elias V Hyttel P Osawa Y Sirotkin A |
| Institution: | 110.Department of Reproduction and Embryology, Research Institute of Animal Production, Kosice, Slovakia ;210.Departments of Pathology, Veterinary University, Kosice, Slovakia ;310.Departments of Physiology, Veterinary University, Kosice, Slovakia ;410.Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Frederiksberg C, Denmark ;510.Endocrine Biochemistry Department, Medical Foundation of Buffalo, New York, USA ; |
| Abstract: | The localization of aromatase, an enzyme converting androgens to estrogen, in the ovaries of superovulated cattle, pigs and sheep was studied immunocytochemically in the preovulatory and postovulatory period using anti-human placental aromatase cytochrome P-450 antiserum. Immunostaining for aromatase was detected in the granulosa cells of preovulatory follicles of all species studied. Theca interna cells were stained in preovulatory follicles in the pig but not in cattle and sheep. Interstitial gland cells, cumulus cells and oocytes were unstained in all species. In cattle and pig the corpora lutea were unstained whereas they displayed staining in the sheep. Preantral and small antral follicles were unstained during both the preovulatory and postovulatory period in all species. It is concluded that granulosa cells of preovulatory follicles are the main residence for aromatase activity in superovulated cattle, pig and sheep, whereas the activity of theca interna and corpora lutea is species specific. |
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