Renalase Protects against Contrast-Induced Nephropathy in Sprague-Dawley Rats |
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| Authors: | Binghui Zhao Qing Zhao Junhui Li Tao Xing Feng Wang Niansong Wang |
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| Institution: | 1. Department of Radiology, Tongji University Affiliated Shanghai Tenth People’s Hospital, Shanghai, China.; 2. Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China.; 3. Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China.; 4. St. Vincent’s Hospital, Melbourne, Australia.; The University of Manchester, UNITED KINGDOM, |
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| Abstract: | BackgroundContrast-induced nephropathy (CIN) is the third leading cause of hospital-acquired acute renal failure. Oxidative stress, apoptosis and inflammation play crucial roles in CIN. Renalase is a newly discovered monoamine oxidase from the kidney. We hypothesize that renalase could protect against CIN through anti-oxidation, anti-inflammation and anti-apoptosis pathways.MethodsWe tested our hypothesis in vivo with a rat model of Ioversol-induced CIN and in vitro. Sprague-Dawley rats were divided into 4 groups (n = 6 per group): control group, Ioversol group (rats subjected to Ioversol-induced CIN), Ioversol plus vehicle group (CIN rats pretreated with vehicle) and Ioversol plus renalase group (CIN rats pretreated with 2 mg/kg recombinant renalase). HK2 cells were treated with Ioversol or H2O2.ResultsThe results showed that pretreatment with renalase attenuated the deterioration of renal function, tubular necrosis, oxidative stress, apoptosis and inflammation (P<0.05). Furthermore, renalase protected HK2 cells against the cytotoxicity of Ioversol and suppressed Caspase-3 activity, oxidative stress and apoptosis induced by H2O2.ConclusionRecombinant renalase protected CIN in rats through anti-oxidation, anti-apoptosis and anti-inflammation mechanisms. |
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