| The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant—free condition |
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| 引用本文： | JIYONGYONG CUIHONGYANG 等.The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant—free condition[J].细胞研究,1995,5(1):67-74. |
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| 作者姓名： | JIYONGYONG CUIHONGYANG |
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| 作者单位： | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031,China [2]ShanghaiInstituteofCellB,ChineseAcademyofSciences,Shanghai200031,China |
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| 摘 要： | Trichosanthin(TCS) is a potent allergen in mice.It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum.TCS can bring out the IgE responses to ovalbumin(OVA),while OVA itself could not induce IgE responses to it .However,TCS only works when OVA immunization is given one day after TCS immunization.Either time lag in OVA immunization,or immunization of both antigens at the same time,or OVA immunization given first,all has no effect on the induction of IgE responses to OVA.Through analysis of the antibody specificity of hybridoma clones,it indicated that specific antibodies to TCS or OVA were secreted by independent B cell clones.The IgE antibldies showed no polyreactivity to different antigens.
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| 关 键 词： | 天花粉蛋白 卵清蛋白 变应原 IgE反应 无佐剂 交叉反应 TCS免疫 |
The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant-free condition |
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| JI YONGYONG, CUIHONG YANG, MING YEH..The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant-free condition[J].Cell Research,1995,5(1):67-74. |
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| Authors: | JI YONGYONG CUIHONG YANG MING YEH |
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| Abstract: | Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovabumin(OVA), while OVA itself could not induce IgE responses to it. How- ever, TCS only works when QVA immunization is given one day after TCS immunization. Either time lag in OVA immunization, or immunization of both antigens at the same time, or OVA immunization given first, all has no effect on the induction of IgE responses to OVA. Through analysis of the antibody specificity of hybridoma clones, it indicated that specific antibodies to TCS or OVA were secreted by independent B cell clones. The IgE antibodies showed no polyreactivity to different antigens. |
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| Keywords: | Trichosanthin ovalbumin allergen IgE response |
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