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Rapid simultaneous lipid extraction and transesterification for fatty acid analyses
Authors:Juan Rodríguez-Ruiz  El-Hassan Belarbi  José Luis García Sánchez  Diego López Alonso
Affiliation:(1) Departamento de Biología Aplicada, Universidad de Almería, Almería, Spain;(2) Departamento de Ingeniería Química, Universidad de Almería, Almería, Spain
Abstract:An improved adaptation of the direct transesterification method of Lepage and Roy (J. Lipid Res. 25, 1391–96, 1984) for the preparation of fatty acid methyl esters allows notable saving of time and reagents. The material being analysed is heated for 10 minutes with methanol, acetyl chloride and hexane. © Rapid Science Ltd. 1998
Keywords:fatty acid  GLC  fatty acid methyl esters  transesterification
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