| 利用杆状病毒表达系统表达金鱼生长激素I基因 |
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| 引用本文： | 林广云 王珣章 Anderson Wong 龙綮新,庞义 余奇理.利用杆状病毒表达系统表达金鱼生长激素I基因[J].生物工程学报,1997,13(2):149-153. |
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| 作者姓名： | 林广云 王珣章 Anderson Wong 龙綮新 庞义 余奇理 |
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| 作者单位： | 1.中山大学生物防治国家重点实验室 广州 510275；2.香港大学动物系 |
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| 摘 要： | 以不含起始密码ATG的质粒Psxivvi+X3为转移载体，将编码金鱼生长激素I的Cdna插入粉纹夜蛾核型多角体病毒(TnNPV)基因组中，构建了形成多角体的重组病毒株TnNPV—SX+gfGHl21a。该毒株能利用合成多角体XIV串联启动子，在重组病毒感染的草地贪夜蛾(Spodoptera frugiperda,Sf)昆虫细胞及银纹夜蛾幼虫中表达金鱼生长激素I基因。感染离体细胞及虫俸后的蛋白SDS聚丙烯酰胺凝胶电泳表明．所表达的蛋白分子量为22．5kDa，与理论计算值相符。Westem印迹证实。金鱼生长激素特异蛋白得到表达。
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| 关 键 词： | 金鱼生长激素．杆状病毒，表达，分泌 |
Expression of Goldfish Growth Hormone I Gene Using Baculovirus Expression System |
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| Lin Guangyun,\ Wang Xunzhang,\ Anderson Wong,\ Long Qingxin,\ Pang Yi,\ Yu Keili.Expression of Goldfish Growth Hormone I Gene Using Baculovirus Expression System[J].Chinese Journal of Biotechnology,1997,13(2):149-153. |
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| Authors: | Lin Guangyun \ Wang Xunzhang \ Anderson Wong \ Long Qingxin \ Pang Yi \ Yu Keili |
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| Institution: | Lin Guangyun 1\ Wang Xunzhang 1\ Anderson Wong 2\ Long Qingxin 1\ Pang Yi 1\ Yu Keili 2 |
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| Abstract: | Using pSXIVVI +X3 without an initiation codon as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding goldfish growth hormone I (gf GHI) under the control of the Syn XIV promoter, has been constructed. Immunoblot analysis showed that the molecular weight of the expressed protein was 22 5kDa as expected, revealed that the virus mediated gfGHI was synthesized in the Sf cells, larvae and secreted into the supernatant and hemolymph. |
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| Keywords: | Goldfish growth hormone baculovirus expression secretion |
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