Epithelial outgrowth from suspension cultures of human prostatic tissue |
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Authors: | Dr Mukta M Webber Oliver G Stonington Patricia A Poché |
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Institution: | (1) Division of Urology, Department of Surgery, University of Colorado School of Medicine, 4200 East Ninth Ave., Box 2539, 80220 Denver, Colorado |
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Abstract: | Summary The primary objective of this study was to obtain pure cultures of prostatic epithelium. Encapsulation by epithelial cells
and hypocellularity in stroma occurred when explants of prostatic tissue were maintained in suspension cultures. Twenty per
cent fetal bovine serum incorporated into the medium provided optimal conditions for encapsulation and preservation of epithelial
cell viability and architecture. Horse serum at the same concentration was less effective.
When encapsulated explants were allowed to attach to the substrate, 10 and 20% horse serum favored growth of epithelial cells
while fetal bovine serum also stimulated fibroblastic growth.
Mechanisms for the induction of hypocellularity, encapsulation, squamous metaplasia and central necrosis in explants were
studied. Relationship between the type and concentration of serum and the nature and extent of outgrowth are discussed.
This work was supported in part by the American Cancer Society Grants DT-20 and IN-5N, U. S. Public Health Service Grant RR-05357,
the Parke Davis Fund, and the John U. White Fund for Urological Research. |
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Keywords: | prostatic epithelium explants encapsulation hypocellularity serum |
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