逍遥散对慢性束缚应激模型大鼠相关脑区CRF基因表达的影响

目的:观察慢性束缚应激大鼠相关脑区CRF mRNA(下丘脑、垂体、海马、皮层)含量变化以及逍遥散对其影响.方法:用RT-PCR和图像分析方法测定相关脑区CRF mRNA含量变化.结果:应激组较正常对照组在下丘脑CRF-1基因表达下调(P<0.01).在下丘脑逍遥散组较应激组CRF-1基因表达显著下调(P<0.01),CRF-2基因表达显著上调(P<0.01);在海马区逍遥散组CRF-2基因表达较模型组上调(P<0.05);在皮层逍遥散组CRF-1基因表达较应激组则显著上调(P<0.01).结论:逍遥散组对慢性束缚应激中枢神经肽CRF的调节位点在下丘脑、垂体、海马和皮层,充分证实逍遥散的调节靶点与下丘脑、边缘系统及皮层中枢有关.

Effect of Xiaoyao powder on changes of relative brain zone CRF gene expression in chronic restrained stress rats

Aim: To observe the effect of Xiaoyao Powder on the content changes of CRF mRNA in relative brain zone (hypothalamus,pituitary,hippocampus,cortex) in chronic restrained stress rats. Methods: RT-PCR and graphic analysis methods were applied to test the content changes of CRF mRNA in relative brain zone. Results: The CRF-1 gene expression in hypothalamus was modulated lower in stress group and the difference was significant when compared with control group(P<0.01).The gene expression of CRF-1 in Xiaoyao Powder group was markedly modulated lower in hypothalamus,but it was markedly modulated higher in cortex as compared with control group (P<0.01). The gene expression of CRF-2 in Xiaoyao Powder group was higher in hypothalamus than that in stress group (P<0.01),and it was also higher in hippocampus as compared with that in control and stress group (P<0.01,P<0.05 respectively). Conclusion: The modulation point of Xiaoyao Powder group on central neuropeptide of chronic restrained stress is respectively hypothalamus,pituitary,hippocampus and cortex. The modulating target is hypothalamus,the limbic system and the cortex center. It can be suggested that medicine of smoothing the liver simultaneously have poly-target and dual-modulating action and been involved in the integration function of nerve-endocrine-immune net.