Ferritin enhances production of DNA strand breaks by 6-hydroxydopamine,ascorbic acid and H2O2 in CCC PM2 bacteriophage DNA |
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Authors: | Achim G Rieth Stephan L Baader Holger N Lode Gernot Bruchelt Dietrich Niethammer |
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Institution: | (1) Children's Hospital, Department of Hematology and Oncology, University of Tübingen, Ruemelinstrasse 23, 7400 Tübingen, Germany |
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Abstract: | Damage of CCC PM2 DNA by 6-hydroxydopamine (6-OHDA) and ascorbic acid (AA), compounds that are both able to release iron from ferritin, was significantly enhanced in the presence of ferritin. H2O2, a product of 6-OHDA autoxidation, did not induce DNA strand breaks in the absence of ferritin and only to a minor extent in the presence of ferritin. DNA damage by 6-OHDA and AA could be reduced by the hydroxyl radical scavenger mannitol, the iron chelator desferrioxamine, and, partly, by a combination of superoxide dismutase and catalase. These inhibitory effects were clearly less pronounced in the presence of ferritin. Ferritin obviously played an important role as a source of iron in the pro-oxidative processes of 6-OHDA and AA. These features might be of importance in cancer therapy since many tumor cells contain elevated ferritin levels. |
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Keywords: | ascorbic acid DNA damage ferritin hydrogen peroxide 6-hydroxydopamine |
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