Essential role of Src suppressed C kinase substrates in endothelial cell adhesion and spreading |
| |
Authors: | Cheng Chun Liu Haiou Ge Haiyan Qian Ji Qin Jing Sun Linlin Shen Aiguo |
| |
Affiliation: | Institute of Nautical Medicine, Nantong University, Nantong 226001, China. |
| |
Abstract: | Integrin-mediated substrate adhesion of endothelial cells leads to dynamic rearrangement of the actin cytoskeleton. Protein kinase C (PKC) stimulates reorganization of microfilaments and adhesion, but the mechanism by which this occurs is unknown. Src suppressed C kinase substrate (SSeCKS) is a PKC substrate that may play an important role in regulating actin cytoskeleton. We found that SSeCKS was localized to focal adhesion sites soon after cell adhesion and that SSeCKS translocated from the membrane to the cytosol during the process of cell spreading. Using small interfering RNAs specific to SSeCKS, we show that RPMVEC cells in which SSeCKS expression was inhibited reduce adhesion and spread on LN through blocking the formation of actin stress fibers and focal adhesions. These results demonstrated SSeCKS modulate endothelial cells adhesion and spreading by reorganization of the actin cytoskeleton. |
| |
Keywords: | Rat pulmonary microvascular endothelial cell Src suppressed C kinase substrates Endothelial cell adhesion Small interfering RNA |
本文献已被 ScienceDirect PubMed 等数据库收录! |