Male-associated polypeptide (MAP) expression in different compartments of the reproductive system of the mussel Mytilus galloprovincialis: immunocytochemical and Western blot study |
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Authors: | M Torrado A T Mikhailov |
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Institution: | (1) CNRS, Laboratoire de Neurobiologie, Neuroendocrinologie des Insectes, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France, FR;(2) Babraham Institute, Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK Tel.: (44) (0) 1223–336678; Fax: (44) (0) 1223–461954, GB |
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Abstract: | The dissociation and maintenance in culture of cells derived from the mushroom bodies of adult crickets (Acheta domesticus) are described. This primary culture was developed in order to investigate maturation and differentiation of mushroom-body
cells including Kenyon cells, the major intrinsic interneurons of mushroom bodies, which have been shown to be involved in
learning and memory in insects. Three distinct cell types were observed, all identified as neural cells on the basis of their
size, morphology and immunocytochemical staining with horseradish peroxidase. These cells appear to correspond to the three
cell types observed in vivo: Kenyon cells, ganglion mother cells and neuroblasts. Some cells showed neurite growth, usually
with long unipolar processes, occasionally with either bipolar or, more rarely, multipolar processes. Neuronal cell bodies
readily formed seals with patch pipettes, allowing stable, whole-cell, patch-clamp electrophysiological recordings. Depolarization
of the cell under voltage-clamp resulted in at least two types of outwardly directed potassium currents: a delayed rectifier-type
of current that was sensitive to tetraethylammonium, and a cadmium-sensitive current with rapid inactivation. Neither type
of current was affected by quinidine, a blocker of potassium currents recorded from pupal honeybee Kenyon cells. Other ionic
currents, which have yet to be characterized, were also observed.
Received: 30 October 1996 / Accepted: 11 July 1997 |
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Keywords: | Mushroom body Kenyon cells Ganglion mother cells Neuroblasts Neurite growth Cell culture Electrophysiology Cricket (Acheta domesticus) |
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