Hydroxyl radical-scavenging property of indomethacin |
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| Authors: | Kailash Prasad Victor A. Laxdal |
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| Affiliation: | (1) Department of Physiology, College of Medicine, University of Saskatchewan, S7N 0WO Saskatoon, Saskatchewan, Canada;(2) Department of Pathology, College of Medicine, University of Saskatchewan, S7N 0WO Saskatoon, Saskatchewan, Canada;(3) Royal University Hospital, S7N 0WO Saskatoon, Saskatchewan, Canada |
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| Abstract: | The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5–10 mmoles/L) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5–600  moles/L) produced increasing inhibition of generation of 2,3-DHBA (7–67%) and of 2,5-DHBA (7–77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner. |
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| Keywords: | hydroxyl radical indomethacin 2,3-dihydroxybenzoic acid 2,5-dihydroxybenzoic acid photolysis of H2O2 hydroxyl radical scavenger |
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