Analysis of population cytokinetics of chick myocardial cells in tissue culture |
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Authors: | W A Clark D A Fischman |
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Institution: | 1. Department of Medicine, The University of Chicago, Chicago, Illinois 60637 USA;2. Department of Anatomy and Cell Biology, Cornell University Medical College, New York, New York 10021 USA |
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Abstract: | The growth of embryonic chick cardiac myocytes and fibroblasts in tissue culture was evaluated by the kinetics of nuclear labeling during continuous exposure to 3H]thymidine. The fraction of mitotically active cells, the mean intermitotic period and the population doubling times were determined in each cell type during 3 weeks in culture. After 24 hr in culture, 90% of the muscle cells were mitotically active with minimal population doubling times of 65 hr. By 17 days in culture only 5% of the myocytes continued to divide with population doubling times greater than 3000 hr. Primarily, the lengthening of doubling times was due to a withdrawal of cells from the mitotic cycle and much less to a lengthening of the intermitotic period. Growth of cardiac muscle cells from embryonic hearts from 4 to 10 days of development was also compared. Muscle cells from younger hearts displayed greater mitotic activity than those from older hearts at equivalent times in culture. |
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Keywords: | To whom all correspondence should be addressed: Department of Medicine The University of Chicago 950 East 59th Street Box 407 Chicago Ill 60637 |
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