| 钙的光释放技术及其在细胞研究中的应用 |
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| 引用本文： | 徐建华,瞿安连,康华光.钙的光释放技术及其在细胞研究中的应用[J].生物化学与生物物理进展,1997,24(2):132-135. |
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| 作者姓名： | 徐建华 瞿安连 康华光 |
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| 作者单位： | 华中理工大学生物物理与生物化学研究所, 武汉 430074;华中理工大学生物物理与生物化学研究所, 武汉 430074;华中理工大学生物物理与生物化学研究所, 武汉 430074 |
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| 摘 要： | Ca2+的光释放技术通过光解作用使预先引入细胞内的光敏感性螯合剂对Ca2+的亲和性改变，从而实现对细胞内游离钙离子浓度的调控，有助于阐明Ca2+作为第二信使对电兴奋性、肌肉收缩、神经分泌等细胞功能的调制作用.
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| 关 键 词： | 光敏感性螯合剂，细胞内钙离子浓度，光释放 |
| 收稿时间： | 1996/4/28 0:00:00 |
| 修稿时间： | 1996/10/25 0:00:00 |
Photorelease Technique of Ca2+ and Its Applications in Cell Studies |
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| XU Jianhu,QU Anlian and KANG Huaguang.Photorelease Technique of Ca2+ and Its Applications in Cell Studies[J].Progress In Biochemistry and Biophysics,1997,24(2):132-135. |
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| Authors: | XU Jianhu QU Anlian and KANG Huaguang |
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| Institution: | Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, China;Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, China;Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, China |
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| Abstract: | Photorelease technique of Ca 2+ can manipulate intracellular calcium concentation (Ca 2+ ] i) by photolyzing specific photosensitive Ca 2+ chelator in the cell to change its affinity with Ca 2+ . This technique is especially useful in elucidating the intracellular messenger role of Ca 2+ in cell functions such as electrical excitability , muscle contraction and secretion, etc. |
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| Keywords: | photosensitive chelators intracellular Ca2+ concentration photorelease |
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