Dual abnormal effects of mutant MITF encoded by Mi(wh) allele on mouse mast cells: decreased but recognizable transactivation and inhibition of transactivation |
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| Authors: | Kataoka Tatsuki R Morii Eiichi Oboki Keisuke Jippo Tomoko Maeyama Kazutaka Kitamura Yukihiko |
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| Affiliation: | Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA. |
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| Abstract: | Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na(+) + K(+))-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na(+) + K(+))-ATPase is expressed on the cellular membrane surface. |
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| Keywords: | (Na+ + K+)-ATPase site-specific antibody flow cytometry immunofluorescence staining positive inotropy |
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