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Biosynthesis of poly (3-hydroxybutyric acidco-3-hydroxy-4-pentenoic acid) from unrelated substrates byBurkholderia sp. |
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| Authors: | M. F. A. Rodrigues L. F. da Silva J. G. C. Gomez H. E. Valentin A. Steinbüchel |
| Affiliation: | 1. Instituto de Pesquisas Technológicas do Estado de S. Paulo S/A-IPT, Caixa Postal 7151, CEP 01064-970, S?o Paulo, Brazil 2. Institut für Mikrobiologie der Georg-August-Universit?t G?ttingen, Grisebachstrasse 8, D-37077, G?ttingen, Germany 3. Institut für Mikrobiologie der Westf?lischen Wilhelms-Universit?t Münster, Corrensstrasse 3, D-48149, Münster, Germany |
| Abstract: | From soil, two strains of Burkholderia sp. were isolated that synthesized and accumulated a copolyester of 3-hydroxybutyric acid and 3-hydroxy-4-pentenoic acid from single, unrelated carbon sources such as surcose or gluconate. Strain IPT77B, for instance, accumulated from gluconate poly(3-hydroxy- butyric acid co-3-hydroxy-4-pentenoic acid) up to 70% (w/w) of the cellular dry matter, and 3-hydroxy-4-pentenoic acid contributed up to 6.9 mol 100/mol constituents. The occurrence of the constituent with the vinyl pendant group in the polyester was confirmed by gas-chromatographic and nuclear magnetic resonance analysis. Received: 28 December 1994/Accepted: 4 January 1995 |
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