Modified arabinogalactans from callus culture <Emphasis Type="Italic">Silene vulgaris</Emphasis> (M.) G. |
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Authors: | E A Günter Yu S Ovodov |
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Institution: | (1) Department of Rheumatology and Internal Diseases, Medical University of Bialystok, M.C. Sklodowskiej 24a, Bialystok, 15-276, Poland;(2) Department of General and Organic Chemistry, Medical University of Bialystok, Bialystok, Poland |
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Abstract: | The cultivation of Silene vulgaris (M.) G. callus culture on the nutrient mediums contained carbohydrates, phytohormones, nitrogen, and phosphate has led to
the modification of the arabinogalactan structure from the cell walls. It was noticed that a sucrose concentration increase
in the cultivation medium led to an increase of the arabinogalactan fragment yield with a molecular weight more than 300 kDa
and a decrease of the yield of fragments with molecular weight less than 300 kDa. The sucrose concentration increase in the
nutrient medium entailed the increase of arabinose and galactose content in the fragment with the molecular weight more than
300 kDa and a decrease in the fragment with a molecular weight of 100–300 kDa. On the nutrient medium containing a mix of
sucrose and arabinose, the yield of the fraction with a molecular weight more than 300 kDa and the amount of arabinose residues
increased, and the yield of minor fragments and the content of arabinose and galactose residues, included in these, decreased.
On the medium containing an increased concentration of 2,4-dichlorphenoxyacetic acid, the yield of high-molecular fragment
and the content of arabinose residues are two times increased. The decreasing of the amount of arabinose and galactose residues
in the fragment with a molecular weight more than 300 kDa was observed at a lack of nitrogen or phosphate in the nutrient
medium. |
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Keywords: | |
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