Simplified procedure for the analysis of 3- and 4-hydroxyproline |
| |
Authors: | Kuang-Tzu Davis Lin |
| |
Institution: | University of Tennessee College of Medicine, Memorial Research Center/Department of Medical Biology, Knoxville, TN 37920 U.S.A. |
| |
Abstract: | A column chromatographic analysis of 3-hydroxyproline (3-Hyp), 4-hydroxyproline (4-Hyp), and γ-carboxyglutamic acid (Gla) is described. The analyses of urine and plasma were performed with a JLC-6AH amino acid analyzer. A 0.15 M sodium citrate buffer, pH 2.1, was used for elution. Urinary Gla, 3-Hyp, and 4-Hyp were among the seventeen peaks eluted before asparti acid. Hyp, Gla, glutamine, and asparagine in plasma were separated by elution with 0.2 M sodium citrate buffer, pH 3.25, containing 10% methanol. This single-column procedure achieves the sequential separation and quantitation of Gla, 3-Hyp, and 4-Hyp in urine as well as plasma, and is applicable to the diagnosis of collager, metabolism disorders. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|