Interactive effect of light,temperature and TDZ on the regeneration potential of leaf discs of <Emphasis Type="Italic">Fragaria x ananassa</Emphasis> Duch |
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Authors: | Amjad M Husaini M Z Abdin |
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Institution: | (1) Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, India |
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Abstract: | This is the first report where shoot regeneration in strawberry cultivar Chandler has been achieved simultaneously through
both somatic embryogenesis and shoot bud formation. Direct somatic embryogenesis was observed in leaf discs which were cultured
on medium containing MS salts + B5 vitamins + 2% glucose + 18.16 μM thidiazuron (TDZ) and given both chilling and dark treatment for 2 wk at 4 ± 2°C followed
by incubation at 25 ± 2°C under 16-h photoperiod for third wk. After 3 wk, these explants were then subcultured on medium
containing MS salts + B5 vitamins + 2% glucose and incubated under 16-h photoperiod at 25 ± 2°C for further growth and development. Direct regeneration
via de novo shoot bud formation was observed in leaf disks which were given dark treatment and were cultured on medium containing MS
salts + B5 vitamins + 2% glucose supplemented with 9.08 μM TDZ. There was a synergistic effect of photoperiod, dark, and chilling treatments
on somatic embryogenesis, whereas chilling treatment had an inhibitory effect on shoot organogenesis. |
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Keywords: | Strawberry TDZ In vitro Organogenesis Somatic embryogenesis Regeneration |
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